Anther culture for haploid and doubled haploid production
نویسندگان
چکیده
منابع مشابه
Colchicine induced embryogenesis and doubled haploid production in maize (Zea mays L.) anther culture
This study involves in vitro androgenesis of <sp...
متن کاملEfficient haploid and doubled haploid production from unfertilized ovule culture of gentians (Gentiana spp.)
Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...
متن کاملcolchicine induced embryogenesis and doubled haploid production in maize (zea mays l.) anther culture
this study involves in vitro androgenesis of zea maysl. via anther culture. combination of two embryoinduction media (imss & ypm) in presence of different colchicine concentrations (0, 100, 200, 250, 300and 400 mg/l ) in the pretreatment medium (iml) andpretreatment duration (0, 3, 6 and 9 days) in twogenotypes (dh5×dh7 and eth-m82) were tested.after colchicine pretreatment, anthers were tr...
متن کاملNovel technologies in doubled haploid line development
haploid inducer line can be transferred (DH) technology can not only shorten the breeding process but also increase genetic gain. Haploid induction and subsequent genome doubling are the two main steps required for DH technology. Haploids have been generated through the culture of immature male and female gametophytes, and through inter- and intraspecific via chromosome elimination. Here, we fo...
متن کاملProduction of doubled haploid plants of carnation (Dianthus caryophyllus L.) by pseudofertilized ovule culture
Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...
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ژورنال
عنوان ژورنال: Plant Cell, Tissue and Organ Culture (PCTOC)
سال: 2010
ISSN: 0167-6857,1573-5044
DOI: 10.1007/s11240-010-9852-z